The aim of this study is to examine the relationship between preservice teachers' attitudes toward graduate education and their levels of academic self-efficacy. This research was conducted using the quantitative research method and the relational survey model. The participants of the study consisted of 340 preservice teachers studying at the faculties of education in various universities located in the Central Anatolia Region of Turkey. The data collection instruments used in this study included the Graduate Education Attitude Scale and the Academic Self-Efficacy Scale. The data were collected online between December 1, 2024, and March 1, 2025. The obtained data were analyzed using descriptive statistics, independent samples t-test, one-way analysis of variance (ANOVA), and Pearson correlation analysis. The findings revealed a positive and significant relationship between preservice teachers' attitudes toward graduate education and their levels of academic self-efficacy. Additionally, significant differences were found based on grade level and department, while no significant differences were observed based on gender.